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Thank you for explaining this for me. I wish you the best of luck with your research. How many secondary antibodies are you looking to purchase? I hope this information has been of help. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. You can see the products at page Please give all of the items a check and let us know which items are best available with Abcam. Could you please help this customer to solve the problem? Blasticidin S HCl R 50mg Please let us have best alternate products for the above at your earliest. The fluorescent secondary antibodies will then allow these organelles to be visualized. As requested, I have issued a free of charge replacement for one vial of ab To check the status of the order please contact our Customer Service team and reference this number. Your name Your email. For future inquiriesI would like to direct you to the search facility on our website www. What kind of filter or lamp are you using? Sincere regards,. I would also appreciate if you can confirm some further details: 1. Permeabilized with 0.{/INSERTKEYS}{/PARAGRAPH} I look forward to receiving your reply. Check with the manufacturer of your microscope if their filters are suitable for those dyes. This way you canexclude the annoying "bleed-over". I hope this information is helpful, and I thank you for your cooperation. We do not have anti Nanog mouse monoclonal that customer is after. I did send her the data sheets and concentration supplied. So what volume of the given stock would I need to use in one experiment? Do the DyLight secondary antibodies, with a supplied concentration of 0. Best regards. Please try the standard protocols listed below and let us know how you get on. Submit a review Submit a question. In the event that a product is not functioning in the species and applications cited on the product datasheet and the problem has been reported within 6 months of purchase , we would be pleased to provide a free of charge replacement, credit note, or refund. We do not sell this chemical. Abreviews Abreviews. The volume of antibody required per experiment would depend on what experiment the customer is hoping to perform, and what volume of diluted antibody the customer usually used in these experiments. I hope this information is helpful to you. Please help. Submit an Abreview. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. Earn rewards! Dear xxxxxx, Below is the reply from my customer: I wish to use the antibodies in a co-localization experiment. To our knowledge, customised protocols are not required for this product. Please do not hesitate to contact us if you need any more advice or information. Using your protocol, what volume of diluted antibody do you usually apply? Publishing research using ab? We have not performed an experiment like this in the lab before and we will have to develop a protocol. Please enter the name of the protein into the search box at the top of the home page. {PARAGRAPH}{INSERTKEYS}Your browser does not have JavaScript enabled and some parts of this website will not work without it. If you have specific questions about the products identified during the search, please do not hesitate to contact me. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. No antibody was detected against non immunoglobulin serum proteins. This is only a starting point and optimisation of these conditions will be required by the end used and their particular experimental conditions. Washing: 1x PBS 3 min 3. Nanog Antibody A sc 7. Immunocytochemistry primary skin fibroblast cells Excellent. Continue Continue. I'm on a very strict budget and I'm looking for the most cost effective way to perform this experiment. Dear All, Sending you attached a list of the tendered items. Thank you for contacting us. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Send me a copy of this email. Using these filters will help you to identify products that have been tested in the applications and species you are interested in. This is the reason why I was wondering what the recommended protocol would be for the DyLight secondary antibodies. Click here to view the general protocols. This will be the case with any secondary antibody. Datasheet SDS. Thank you for your enquiry and interest. If the customer could please answer the following questions I can help in their request: 1. Please let us know so that we can cite the reference in this datasheet. 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